Coding
Part:BBa_K3617008:Design
Designed by: David Nørgaard Essenbæk Group: iGEM20_UCopenhagen (2020-10-21)
GPA1 with TEV protease cleavage sequence 1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The cleavage sites where chosen by using an alignment of bovine G-α and GPA1 and inspecting the crystal structure of bovine G-α and at the same time taking into consideration residues and regions that have been investigated in mutagenesis studies.
Source
GPA1 from S. cerevisiae.